ea0034s1.1 | GNRH biology ‐ from pulses to longevity (Supported by <emphasis role="italic">Journal of Molecular Endocrinology</emphasis>) | SFEBES2014
Perrett Rebecca
, Voliotis Margaritis
, Armstrong Stephen
, Pope George
, Tsaneva-Atanasova Krasimira
, McArdle Craig
GnRH acts via Gq/11-coupled GPCRs on gonadotropes to control synthesis and secretion of LH and FSH. We use mathematical and statistical approaches as well as automated cell imaging to explore this system. In cells expressing ERK2GFP and GnRH receptors, GnRH pulses cause rapid and transient nuclear translocation of ERK2GFP, providing a live-cell readout for ERK activation1. A mathematical model trained against this data predicted greater sensitivity to re...